The gene editing revolution achieved by the development of CRISPR technologies was possible due to its efficiency and easy manipulation of a programable guide RNA that identify the specified target site. Insertion sequences (IS) from the IS110 and IS1111 families insert the DNA in a targeted and precise directional manner. We have demonstrated that the unique transposase from both IS families is small (˜340aa) and co-purifies with a programable seekRNA (˜78nt) derived from the non-coding region (NCR) within the IS. The seekRNA is essential for both formation of mini-circles and transposition to a target site. We have demonstrated the versatile use of the system as a standalone DNA insertion tool with all the desirable features required to translate it into a widely use genome manipulation tool.