Leo Dr Pinheiro
Dr. Leonardo (Leo) Pinheiro is a Senior Scientist at the Australia’s National Measurement Institute (NMIA) where he has key role on new initiatives of Biometrology group for the development and validation of high accuracy methods for the production of novel nucleic acid reference materials.
Leo has been working with PCR technology for over 25 years but is a confessed travel addict and in past has worked as an author for Lonely Planet Publications.
Scientific Publications
Publications
• Kerry R. Emslie, Jacob L H McLaughlin, Kate Griffiths, Michael Forbes-Smith, Leonardo Pinheiro, Daniel Burke (2019) Droplet volume variability and impact on digital PCR copy number concentration measurements Analytical Chemistry 2019 , 91,6, 4124-4131
• Leonardo Pinheiro and Kerry R. Emslie (2018) Basic Concepts and Validation of Digital PCR Measurements. In: Karlin-Neumann G., Bizouarn F. (eds) Digital PCR. Methods in Molecular Biology, vol 1768. Humana Press, New York, NY
• Leonardo Pinheiro, Helen O’Brien, Julian Druce, Hongdo Do, Pippa Kay, Marissa Daniels, Jingjing You, Daniel Burke, Kate Griffiths, and Kerry R. Emslie (2017) Interlaboratory Reproducibility of Droplet Digital Polymerase Chain Reaction Using a New DNA Reference Material Format. Analytical Chemistry 2017 89 (21), 11243-11251
• P105611.AU Australian Provisional Patent 2017. Reference Array. Leonardo Pinheiro, Kerry Emslie, Kate Griffiths, Michael Forbes-Smith, Daniel Burke
• Philippe Corbisier, Leonardo Pinheiro, Stephane Mazoua, Anne-Marie Kortekaas, Pui Yan Jenny Chung, Tsvetelina Gerganova, Gert Roebben, Hendrik Emons, Kerry Emslie (2015) DNA copy number concentration measured by digital and droplet digital quantitative PCR using certified reference materials. Anal Bioanal Chem 407(7)1831-40.
• Francine Z marques, Priscilla R Prestes, Leonardo Pinheiro, Katrina Scurrah, Kerry R Emslie, Maciej Tomaszewski, Stephen B Harrap, Fadi J Charchar. (2014) Measurement of absolute copy number variation reveals association with essential hypertension. BMC Med Genomics 15;6:44.
• Leonardo Pinheiro and Kerry R. Emslie (2016). Production and assignment of property values for toxic marine phytoplankton Dinoflagellates DNA reference material NMI NA026 (NMI’s internal report).
• Leonardo Pinheiro and Kerry R. Emslie (2016). Production and assignment of property values for Cyanobacteria 200K DNA reference material NMI NA026 (NMI’s internal report).
• Leonardo Pinheiro and Kerry R. Emslie (2014). Production and assignment of property values for DNA reference materials NMI NA012, NMI NA013, NMI NA014, NMI NA015, Cyanobacteria 16S, MCYE/NDAF, CYRA, SXTA (NMI’s internal report).
• Leonardo Pinheiro and Kerry R. Emslie (2014). Production and assignment of property values for NMI NA004, Cyanobacteria 16S, MCYE/NDAF, CYRA, SXTA DNA reference material (NMI’s internal report).
• Leonardo Pinheiro and Kerry R. Emslie (2013). Report of Analysis for Reference Material NMIA NA010-B1 Human Genomic DNA (NMI’s internal report).
• Leonardo Pinheiro and Kerry R. Emslie (2013). Report of Analysis for Reference Material NMIA NA009 DNA copy Number verification Plate – 46% GC (NMI’s internal report).
• Leonardo Pinheiro and Kerry R. Emslie (2013). Report of Analysis for Reference Material NMIA NA008 DNA copy Number verification Plate – High GC (NMI’s internal report).
• Leonardo Pinheiro and Kerry R. Emslie (2013). Development of a synthetic DNA reference material for the validation of quantitative qPCR assays for detection of toxin producing strains of Cyanobacteria (NMI’s internal report).
• Burke DG, Dong L, Bhat S, Forbes-Smith M, Fu S, Pinheiro L, Jing W, Emslie KR (2013) Digital polymerase chain reaction measured pUC19 marker as calibrant for HPLC measurement of DNA quantity. Anal Chem.85(3):1657-64
• Pinheiro LB, Coleman VA, Hindson CM, Herrmann J, Hindson BJ, Bhat S, Emslie KR. (2012) Evaluation of a Droplet Digital Polymerase Chain Reaction Format for DNA Copy Number Quantification Anal Chem.84(2):1003-11.
• Pinheiro, L.B., Gibbs, M.D., Vesey, G., Smith, J. and Bergquist, P.L. (2008) Fluorescent reference strains of bacteria by chromosomal integration of a modified green fluorescent protein gene. Appl Microbiol Biotechnol. 77:1287-95.
• Patent (WO/2006/029449) CHROMOSOMAL INSERTION OF GFP INTO BACTERIA FOR QUALITY CONTROL PINHEIRO, Leonardo, B.; (AU). BERGQUIST, Peter; (NZ).GIBBS, Moreland; (AU). VESEY, Graham; (AU)
• Australian Provisional Patent 2006. Nucleic acid controls. PINHEIRO, Leonardo, B.; (AU). BERGQUIST, Peter; (NZ).GIBBS, Moreland; (AU). VESEY, Graham; (AU)
• Development of a generic inhibition control for real-time PCR assays. Poster resented at International qPCR conference September 2005 Freising Germany.
• Development of Fluorescent Reference Bacterial Strains by Chromosomal Integration of a modified Green Fluorescent Protein Gene. Poster presented at International Association of Food Protection. Baltimore, August 2005.
• Pinheiro, L. B. and Scopes R. K. Thermostable Variants of Zymomonas mobilis Alcohol Dehydrogenase Obtained Using PCR-Mediated Random Mutagenesis. Protein Expression and Purification. 1998. 6:61-66.
Other Publications
As Travel Guidebook Writer for Lonely Planet Publications 1995 – 2003.
Co-author of the update of Brazil - a travel survival kit, 3rd, 4th and 5th editions, Travel with children 4th edition and the Fiji and Tuvalu chapters of South Pacific 1st edition. Co-author the full re-write of Fiji – a travel survival kit, 4th edition and updates of the subsequent 5th and 6th editions.
Presentations in Scientific Forums
• 1st Asia Pacific Droplet Digital PCR Symposium – Application of Digital PCR for the Development of DNA reference Materials, Bangkok Thailand, October 2016.
• Advances in digital PCR workshop presentation – Development of DNA Reference Materials for verification of digital PCR measuring systems – Sydney Australia, May 2015.
• Advances in digital PCR workshop presentation – Development of DNA Reference Materials for verification of digital PCR measuring systems – Melbourne Australia, March 2015.
• NMI symposium - Development of PhytoxigeneTM CyanoDTec: A rapid molecular assay for the
routine monitoring and differentiation of toxin-producing cyanobacterial blooms, NMI Linfield Australia, March 2015.
• National Cyanobacteria Workshop presentation – Development of CyanoDTec; A rapid molecular for the routine monitoring of toxic cyanobacterial blooms, Adelaide Australia, September 2014.
• Conference presentation – What Validation is Required for Digital PCR Measurements? Digital PCR technology & Tools for Precision Diagnostics, San Diego USA, October 2013.
• BioRad Annual ddPCR users group presentation - DNA Reference Materials for verification of digital PCR measuring systems, San Diego USA, October 2013.
• BioRad Digital Biology Centre presentation - DNA Reference Materials for verification of digital PCR measuring systems, Pleasanton USA, October 2013.
• Development of a generic inhibition control for real-time PCR assays. Presented at International qPCR conference September 2005 Freising Germany.
• Development of Fluorescent Reference Bacterial Strains by Chromosomal Integration of a modified Green Fluorescent Protein Gene. Presented at International Association of Food Protection. Baltimore, August 2005.